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Figure 5 | Molecular Cancer

Figure 5

From: Silencing of Apoptosis-Inducing factor and poly (ADP-ribose) glycohydrolase reveals novel roles in breast cancer cell death after chemotherapy

Figure 5

MDA-MB-231 cell death after silencing of PARG, inhibition of caspases, and RNAi knockdown of AIF. MDA-MB-231 cells were pretreated with Q-VD-OPh and transfected with siRNA for PARG and AIF. A, Cell death was quantified by FACS after treatment with 0.5 mM MNNG for 45 min. B, The percentage of cells that exhibited propidium iodide (PI), annexin V-FITC, or dual PI/annexin V-FITC fluorescence from FACS analysis in B were determined. C, Extracts from cells in A were probed for the immunoblotting detection of PARG, AIF, and procaspase-3. SDS-PAGE loading controls were provided by the immunoblotting detection of β-actin. All error bars represent the SEM. *P < 0.01 between Scr vs. Scr + Q, PARG vs. PARG + Q, PARG + Q vs. PARG + Q + AIF, PARG + Q + AIF vs. PARG, and PARG + Q + AIF vs. Scr + Q (one-way ANOVA and unpaired Student’s t-test).

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