MACC1 SNPs L31V, S515L, and R804T and MACC1 expression in colorectal cells and tumors A). MACC1 mRNA expression was determined in 60 colorectal tumors by qRT-PCR. Box plots show the mRNA expression compared to MACC1 genotypes. SNPs have no effect on the MACC1 mRNA expression in these colorectal tumors. B) SW480, DLD1, HCT116 colorectal cancer cells were transfected with pcDNA3.1/MACC1/wt, pcDNA3.1/MACC1/L31V, pcDNA3.1/MACC1/S515L and pcDNA3.1/MACC1/R804T. The MACC1 mRNA expression in these cell clones was measured by qRT-PCR and normalized to the MACC1 mRNA expression of parental cells. Additionally, MACC1 protein expression was determined. β-tubulin was used as a loading control. SNPs have no effect on the MACC1 mRNA and protein expression in SW480, DLD1 and HCT116 colorectal cancer cells.