MACC1 SNPs L31V, S515L, and R804T, cell motility, and proliferation in colorectal cancer cells. A) SW480, DLD1 and HCT116 colorectal cancer cells were transfected with pcDNA3.1/MACC1/wt, pcDNA3.1/MACC1/L31V, pcDNA3.1/MACC1/S515L and pcDNA3.1/MACC1/R804T. Cell migration was analyzed using Boyden chamber assay. SNPs have no effect on the migratory abilities of the cells. B) The proliferation of SW480, DLD1, HCT116 and transfected cells was analyzed by MTT assay. SNPs do not affect the proliferative potential of the cells. C) For the wound healing assay SW480, DLD1, HCT116 and transfected cells were seeded and scratched. The wound healing area was documented until day 4. Cells with MACC1 SNPs show the same abilities to close the wound compared to cells with wild-type MACC1.