Figure 5

miR-31 promoter is heavily methylated in the basal TN versus the luminal BC cell lines. (A) Methylation specific PCR [MSP] of bisulfite-modified DNA of the indicated cell lines using 2 sets of primers. (B) Ratio of overall methylated over unmethylated CpGs by sequencing of bisulfite-modified DNA from the indicated cell lines. The location of the MSP and bisulfite sequencing primers is shown in Figure 1C.