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Figure 1 | Molecular Cancer

Figure 1

From: Targeting filamin A reduces K-RAS–induced lung adenocarcinomas and endothelial response to tumor growth in mice

Figure 1

Strategy for inactivating the expression of FLNA and simultaneously activating the expression of K-RASG12D in mouse lungs. (A) Schematic illustration of mouse allele, Flnafl and Kras2LSL. Numbered boxes represent exons. Infection of lungs with adenoviral vector encoding Cre (Ad-Cre) yields a truncation of exons 2–8 of Flna and removal of LSL before constitutive activation of the Kras2 promoter. Sites for forward (F) and reverse (R) oligonucleotide primers are indicated (arrows). (B) Genotyping of male (Flnao/fl) and female (Flna+/fl) mouse tails by genomic PCR using F1 and R1 primers detecting Flna alleles. Higher band represents Flnafl allele. (C) Genotyping of mouse tails by genomic PCR using F4 and R4 primers for LSL. Amplicons represent Kras2LSL.

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Molecular Cancer

ISSN: 1476-4598