Figure 5
Fibrosarcoma cells inoculated into mice lacking Flna in endothelial cells form smaller tumors. (A) Immunoblot of endothelial cells extracted from VE-CadCre/+Flnao/+ and VE-CadCre/+Flnao/fl lungs. Actin loading served as internal loading. (B) Decreased percentage of migrated VE-CadCre/+Flnao/fl endothelial cells as assayed by a modified Boyden’s chamber. Student’s t test, *P < 0.05. (C) Tumor size measurements up to 13 days after inoculation of mouse fibrosarcoma cells into VE-CadCre/+Flnao/+ and VE-CadCre/+Flnao/fl male mice. Student’s t test, *P < 0.05. (D) Representative micrographs of vascular networks in VE-CadCre/+Flnao/+ and VE-CadCre/+Flnao/fl fibrosarcomas. Red color represents PECAM-positive endothelial cells, whereas blue color identifies NG-2–positive pericytes. Original magnifications × 20. (E) Quantification of microvascular and pericyte areas, and percentage of pericyte-covered vascular structures within tumors. Student’s t test, *P < 0.05.