Figure 3
From: Promoter Methylation status of HIN-1 associated with outcomes of ovarian clear cell adenocarcinoma
(A) Summary of bisulphite sequence results of HIN-1 in OCCA cell lines. The specific CpG sites detected by MS-PCR were almost completely in accordance with bisulphite sequencing of HIN-1. (B) Promoter methylation of HIN-1 in OCCA cell lines detected by MS-PCR. (C) Summary of bisulphite sequence results of HIN-1 in OCCA tissues. (D) Promoter methylation of HIN-1 in OCCA cell lines detected by MS-PCR. (E) The mRNA expression of HIN-1 correlated well with the methylation status in OCCA cell lines and cancerous tissues, ovarian serous cancers, and benign endometriotic cysts. (F) Representative figures between the methylation status and protein expression of HIN-1 by Western blot analysis. Samples 55 and 68 were OCCA tissues with HIN-1 methylation. Samples 50, 86, 04, and 08 were OCCA tissues without methylation. Samples B30 and B31 were benign endometriotic cysts. (G) Immunohistochemical staining of HIN-1. E1: Endometriotic cyst tissue (40x10), E2: HIN-1 overexpression from OCCA (sample 68 with unmethylated HIN-1, 40x10), E3: loss of HIN-1 expression from OCCA (sample 50 with methylated HIN-1, 40x10).