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Figure 3 | Molecular Cancer

Figure 3

From: Interferon regulatory factor 4 binding protein is a novel p53 target gene and suppresses cisplatin-induced apoptosis of breast cancer cells

Figure 3

Recruitment of the p53 protein to the IBP promoter. (A) EMSA assay demonstrating the ability of p53 to bind the IBP promoter in vitro. The binding reactions were performed with nuclear extracts and various labelled oligonucleotides in the presence of unlabelled probes or antibodies as indicated. HCT116 p53−/− nuclear proteins were extracted as a negative control. A p21 probe served as a positive control. The band was supershifted using an antibody against p53. Mouse IgG served as a supershift negative control. (B) ChIP assay was performed using an anti-p53 antibody to test whether the p53 protein could bind the IBP promoter in HCT116 p53+/+, HCT116 p53−/− and MCF-7 cells. Cells were treated with 50 nmol/L doxorubicin for 8 h. Mouse IgG was used as a negative control.

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Molecular Cancer

ISSN: 1476-4598