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Figure 7 | Molecular Cancer

Figure 7

From: Interferon regulatory factor 4 binding protein is a novel p53 target gene and suppresses cisplatin-induced apoptosis of breast cancer cells

Figure 7

IBP over-expression activates p53 pathway though AKT. (A) The relative basal expression levels of IBP, p53, p21, Bcl-2, and Bax in the IBP-knockdown MCF-7 cells (IBP-RNAi) and IBP-over-expressing MCF-7 cells (IBP). (B) The expression of p53 Ser-15 phosphorylation was tested in IBP-over-expressing MCF-7 cells and IBP knockdown cells treated with cisplatin for 24 h. (C) Western analysis of levels AKT Ser-473, MDM2 Ser-166 phosphorylation in IBP-over-expressing MCF-7 cells and control cells. (D) The expression of MDM2 Ser-166 phosphorylation, p53 and p21 were detected in IBP-over-expressing MCF-7 cells treated with Ly294002 or wortmannin for 24 h. GAPDH served as a loading control.

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Molecular Cancer

ISSN: 1476-4598