Figure 1

Activated Akt signaling, regardless of isoform type, attenuates expression of EMT-associated transcripts and proteins in MCF10A cells. A. Cells were infected with either control retrovirus Babe (V) or with one expressing Akt1 (1), Akt2 (2) or Akt3 (3), respectively. The total RNA was extracted from the respective cells and subjected to RT-qPCR to quantify the transcripts of interest. Heatmap represents relative mRNA levels of EMT-associated transcripts in Akt-infected cells compared to the ones in control counterpart. B. MCF10A cells were lyzed and the resultant mixture was subject to Western immunoblotting using indicated antibodies to assess the levels of proteins of interest. Similar level of GAPDH expression was an indicative of nearly equal amounts of proteins introduced in all samples.