Figure 5
Activated Akt signaling decreases breast stem/progenitor cell subpopulations. MCF10A cells were infected with retroviruses to respectively express empty vector (Babe), Akt1, Akt2, or Akt3 and then subject to PE-CD24 and FITC-CD44 double-staining (A), ALDEFLUOR assay (B), or serially passaged mammosphere formation assay from which mammospheres were counted under a microscope by independent investigators (C). * Denotes that the number of mammospheres obtained from Akt-expressing cells differed significantly (p < 0.05) from the vector controls.