The inhibitory effects of Akt signaling on EMT, cell motility and stem/progenitor cell subfraction can be attenuated in cells that become progressively malignant. A. MI, MII and MIII cells were infected with retroviruses expressing vector control (Babe), Akt1 (1), Akt2 (2), or Akt3 (3), followed by RT-qPCR analysis for assessing expression levels of EMT-associated transcripts. Heatmap represents relative mRNA levels of various transcripts in Akt-expressing cells compared to the ones in control cells set as 1. B. Infected MI, MII, or MIII cells were tested for migration assay 12 hours after seeding on the top chamber of transwell plate and * denotes a significant change (p < 0.05) as compared to the control cells (Babe). C. Infected MI, MII or MIII cells were subjected to ALDEFLUOR staining (upper) or PE-CD24 plus FITC-CD44 double-staining (lower), followed by FACS analysis to quantify the frequency of ALDH+ and CD44+/high/CD24-/low subpopulations. * denotes significant (p < 0.05) difference between the Akt-expressing cells and the counterpart control.