Figure 1
From: Rab31 expression levels modulate tumor-relevant characteristics of breast cancer cells
Characterization of polyclonal antibodies directed to human rab31. (A) The reaction pattern of polyclonal antibody (pAb) RT3-IgG (black) and of the pre-immune rabbit serum (gray) was analyzed by a ‘one-sided ELISA’ assay[19]. rab31-His, purified recombinant, histidine-tagged rab31, used for immunization; GST-rab31, purified recombinant GST-rab31 fusion protein; BSA, bovine serum albumin. (B) In Western blot analyses, pAb RT3-IgG strongly reacts with recombinant rab31 and not at all or only weakly with the highly homologous Rab proteins rab5 and rab22A, respectively. (C) Immunohistochemical staining of paraffin-embedded, formalin-fixed breast cancer specimens with pAb RT4-IgG. Specific immunostaining is observed in the cytoplasm as well as in the nucleus of cancer cells (upper panel, see arrow), stromal cells are stained less frequently (lower panel, see arrow).