Figure 3

Rab31 overexpression enhances proliferation of breast cancer cells in vitro. Cells were seeded in triplicate onto 24-well plates, detached with 0.05% EDTA-solution after 24, 48, 72 and 96 h of cultivation and counted with a Neubauer-chamber under trypan blue exclusion. Cell number at 24 h was set to 100%; increase in cell number was expressed relative to the 24 h value in %. Mean values (± SEM) of at least three independent experiments are depicted. Statistically significant differences (p < 0.05) to the vector control group are indicated by an asterisk. (A) Only MDA-MB-231 cell clones with a homogenous high rab31 expression show a significant increase in cell proliferation compared to vector control cells. (B) CAMA-1 batch-transfected cells, as well as CAMA-1 cell clones with homogenous medium and high expression of rab31, respectively, display a significant increase in cell proliferation compared to vector control cells after 96 h of cultivation.