Figure 5

Rab31 overexpression reduces the invasive capacity of cells in vitro. Stably transfected MDA-MB-231 cells were seeded into the upper compartments of Matrigel™-coated invasion chambers. After 24 h of incubation, invaded cells were fixed, stained, and counted. At least three independent experiments were performed in triplicates each. Results are given in %, normalized to the number of invaded vector-transfected cells. Whisker box plots indicate the 25th and 75th percentile, the vertical bars indicate the 10th and 90th percentile. The median value of at least three independent experiments is indicated by a bar within the box. Statistically significant differences (p < 0.05) to the vector control group are indicated by an asterisk, differences between other groups are indicated by a line with an asterisk.