Figure 5
From: Cooperative interaction of MUC1 with the HGF/c-Met pathway during hepatocarcinogenesis
Activation of c-Met signaling pathway by HGF administration induced p42/44-MAPK and β-catenin phosphorylation. Overnight starved Mahlavu (A, B) and SNU-449 (C, D) cells were stimulated with medium alone and with HGF for 15, 30, and 60 min. Total cell lysates were then analyzed with Western blotting. Blots were probed with anti-p-Met, anti-c-Met, anti-MUC1, p-p42/44-MAPK, p42/44-MAPK, anti-p-β-catenin, anti-β-catenin, anti-c-Myc, and anti-calnexin antibodies.