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Figure 6 | Molecular Cancer

Figure 6

From: Cooperative interaction of MUC1 with the HGF/c-Met pathway during hepatocarcinogenesis

Figure 6

SU11274 selectively inhibited c-Met phosphorylation and downstream signaling. Mahlavu cells were incubated with medium alone or with SU11274 overnight. Then cells were treated with or without HGF for 60 min. Total protein lysates were analyzed by immunoblotting. Membranes were blotted with anti-p-Met, anti-c-Met, anti-MUC1 (A), anti-p-β-catenin, anti-β-catenin, anti-c-Myc (B), and anti-calnexin antibodies. SU11274 pretreated cells and control cells were seeded in the upper chamber of Boyden chambers. No HGF or 40 ng/ml HGF was added to the lower chamber. Following 24 h incubation, cells that had migrated or invaded onto the lower surface were stained and counted. Bars represent mean number ± S.E. of migrating (C) or invading (D) cells (* indicates p < 0.05, NS: not significant).

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