Consequences of MUC1 silencing on downstream signaling molecules. Mahlavu cells were transfected with siRNA targeted to MUC1 for 72 h followed by 60 min HGF stimulation. Controls included no treatment and MUC1 siRNA-treated cells. Total protein lysates were analyzed by immunoblotting. Membranes were blotted with anti-p-Met, anti-c-Met, anti-MUC1, anti-p-β-catenin, anti-β-catenin, anti-c-Myc, and anti-calnexin antibodies.