Skip to main content

Advertisement

Figure 2 | Molecular Cancer

Figure 2

From: The small GTPase RhoG mediates glioblastoma cell invasion

Figure 2

RhoG mediates HGF-induced Rac1 activation. A) Time-dependence of RhoG activation by HGF. SNB19 cells were serum starved for 24 hours, and incubated further with or without HGF for the indicated time. Subsequently, cells were lysed and RhoG activity was determined using an active RhoG pull down assay as detailed in Materials and Methods. Western blot shows activated RhoG and total RhoG for HGF (25 ng/ml)-stimulated and control cells. Histogram shows quantification (+/− SEM) of at least 5 independent experiments. B) RhoG mediates HGF-induced Rac activation. Cells were transfected with siRNAs (10 nM) directed against luciferase (control) or RhoG-2. Forty eight hours after transfection, cells were serum starved for 24 hours, and incubated further with or without HGF for the indicated time periods. Rac activity was determined using the Rac G-LISA Activation Assay as detailed in Materials and Methods. The results shown represent the means +/− SEM of at least 3 independent experiments (* = p < 0.05, two-tailed t test). C) depletion of RhoG does not affect expression of Rac1 nor vice versa. Cells were transfected with siRNAs (10 nM) directed against luciferase (control) or RhoG (two different oligos). Cells were lysed 72 hours after transfection, followed by western blot analysis. Histograms shows quantification (+/− SEM) of between 3 and 4 independent experiments.

Back to article page