The expression of coronin 3 in gastric cancer tissues and cell lines. A. Immunohistochemical analysis of coronin 3 expression in gastric cancer and lymph lode metastasis tissues. a, primary gastric cancer tissues; b, lymph lode metastasis tissue; c, serial section with primary gastric cancer tissue (a) from the tissue array, negative control (pre-immune serum staining). B. Expression of coronin 3 in gastric cancer cell lines. β-actin was used as the internal control. The level of coronin 3 protein expression was significantly higher in the highly invasive MKN28-M cell subline than in the less invasive cell subline MKN28-NM. The level of coronin 3 mRNA expression was consistent with that of Coronin 3 protein expression.