Figure 3

The effects of Coronin 3 on the migration and invasion of gastric cancer cells. The representative results of three similar experiments are shown. A and B. The MKN45 cells were infected with shRNA-LV, and the MKN28-NM cells were stably transfected with pcDNA3.1-Coronin 3. The protein and mRNA expression of Coronin 3 were then evaluated by Western blotting and qPCR. β-actin was used as the internal control. The MOCK samples were treated with scrambled shRNA-LV or the pcDNA3.1 vector. C. The migratory ability of the cells was evaluated with a wound-healing assay. The wound widths were measured at time 0 and 24 h after wounding, and the closure ratio was calculated in accordance with the following formula: wound closure (%) = (width 0 h) - width 24 h) / width 0 h. * p < 0.05. These results were then compared to those of the control cells. D. The invasive ability was evaluated by counting the number of cells that had invaded the Matrigel and the 8-μm-pore Transwell membrane. *, p < 0.05, compared to the cells infected with scrambled shRNA-LV or transfected with the pcDNA3.1 vector.