In situ hybridization detection of miR-146a expression in the gastrin KO mouse gastric cancer model and human gastric cancer. (A) Expression of miR-146a was not detected in WT fundic mouse tissue, (B) while miR-146a-positive cells (blue nuclei) were detected in the metaplastic fundic tissue from gastrin KO mice. (C) No signal was detected with the scrambled LNA probe. (D) miR-146a expression was absent in normal human gastric tissue, (E) but present in human gastric adenocarcinoma cells (blue nuclei). (F) Negative control using a scrambled LNA probe. Representative miR-146a-positive cells are indicated by arrows. Original magnification x20, Scale bar = 100 μm.