miR-146a targets COPS8 and CARD10 GPCR-mediated NF-κB activation. SNU638 cells were co-transfected with NF-κB reporter plasmid and Renilla luciferase control plasmid along with control (siGlo), siRNA or miR-146a. Cells were stimulated with 25 μM LPA and luciferase activity was measured. Luciferase activity is given relative to activity in control transfected LPA-stimulated cells. siRNA against CARD10 and COPS8, miR-146a, miR-146a inhibitor and a siRNA mix reduced luciferase activity. siRNA against IRAK1 and TRAF6 did not reduce luciferase activity. LPA-stimulated NF-κB activation was also inhibited by siRNA against COPS2 and COPS5, demonstrating that other perturbation of the COP9 signalosome complex reduces GPCR-mediated NF-κB activation. siMix, mix of siRNAs against CARD10, COPS8, IRAK1 and TRAF6. Data are shown as the mean ± S.D. of seven biological replicates.* = P < 0.05. The relative expression of TRAF6, COPS8, IRAK1, CARD10, COPS2 and COPS5 was measured using qPCR. Gray-shading indicates a significant change in expression, p < 0.05, n = 4.