Figure 1

CEACAM6 expression in HNSCC cell lines and patient tumours. A) RtPCR analysis of CEACAM6 mRNA expression in subconfluent cultures ofHNSCC cell lines and normal HEKs (n = 4). High tumour initiating (TI) activity refers to cell lines that require less than 10⁴ cells to form a tumour and low TI activity refers to cell lines that require greater than 3x10⁴ cells to form a tumour in NOD/SCID mice. B) Comparison of CEACAM6 mRNA expression between high TI and Low TI cell lines (n = 4). C) CEACAM6 expression in a xenotransplanted tumour derived from the Detroit 562 cell line and the staining pattern for CEACAM6 in a normal oral mucosal sample. D) CEACAM6 expression in HNSCC patient tumours. Arrows point to areas of focal expression of CEACAM6. All images taken at 20x magnification (boxed inserts are IgG control sections). All data presented as mean +/− sem. * refers to P ≤ 0.05.