Figure 4
Knock down of CEACAM6 in the Detroit 562 cell line. A) The mRNA expression of CEACAM6 in control miR RNAi and 2 miR RNAi knock down sequences (miR CEA and miR CEA Dux) was quantified by rtPCR analysis. B) The knock down of CEACAM6 using miR CEA Dux was confirmed at the protein level using western blot analysis. C) BrdU incorporation in control (miR Control) and knock down detroit cells (miR CEA Dux) is shown (n = 6). BrdU is reported as Absorbance Units/well (A450nm-A560nm). D) Percentage of total cells that express Annexin V positivity in control (miR Control) and knockdown Detroit cells (miR CEA Dux) is shown. Data presented as mean +/− sem of at least 3 independent experiments performed in triplicate. * P ≤ 0.05.