P276-00 mediated cytotoxic effect in MCL cell lines: Dose and time dependent analysis. The effect of P276-00 on viability of MCL cells was determined by CCK8 assay. Mantle cell lymphoma cell lines (A) Jeko-1, (B) Mino, and (C) Rec-1 were cultured in the presence of increasing concentrations of P276-00 (0.01-3 μmol/L) for 48 and 96 h. P276-00 showed IC50 of 0.35 μmol/L at 48 hr and 0.22 μmol/L at 96 hr in Jeko-1 cells. In Mino and Rec-1 the IC50 were 0.5 μmol/L at 48 hr and 0.21-0.33 μmol/L at 96 hr (Table 1). Data presented as the average ± SE of three independent experiments.