Figure 1

Unique microRNA signature of NPM1^mut AML is characterised by miR-10a over-expression. A Hierarchical cluster analysis of 28 AML samples according to NPM1 mutational status, with over-expression of miR-10a, let-7b, let-7c and under-expression of miR-130a and miR-335, with accompanying fold change B. C Validation of miR-10a expression by qRT-PCR in normal bone marrow, NPM1^wt- AML and NPM1^mut-AML respectively, with values presented normalised to the mean of the normal bone marrow samples. D miR-10a expression by qRT-PCR in selected malignant cell lines: MV4-11, OCI-AML3 (myelomonoblastic) and THP-1 (monoblastic). The expression values are depicted relative to that of MV4-11 cells, which had the lowest miR-10a expression. Error bars denote SEM. *p < 0.05 by Unpaired Two-tailed T-test.