Figure 1
From: Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence
Hypoxia induced HIF-1α nuclear translocation in TG1-1 cells is cyclical. Western blots of TG1-1 nuclear (N) and cytoplasmic (C) lysates show induction and nuclear translocation of HIF-1α when cultured with 100μM CoCl2 (A) or in nuclear lysates of cells cultured in 1% O2 (B) with TBP as a nuclear loading control and L as a ladder lane. TG1-1 cells were also left untreated (left panel) or treated with CoCl2 (right panel) for 24 hours and immunofluorescently stained for VEGF (green), HIF-1α (red) and DAPI for nuclear staining (blue) and representative pictures of HIF-1α staining in TG1-1 cells also demonstrate nuclear translocation in treated cells (C).