Skip to main content
Figure 6 | Molecular Cancer

Figure 6

From: Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence

Figure 6

Estrogen induced in vitro vasculogenesis is sensitive to anti-hypoxic and antiestrogenic agents. TG1-1 cells were treated with estrogen in combination with the antiestrogen Fulvestrant, the anti-hypoxic agent YC-1, or 50μg/mL of the generic protein synthesis inhibitor cycloheximide. Western blot analysis of nuclear lysates demonstrated inhibition of estrogen induced HIF-1α translocation by cycloheximide (A). Cells were again treated as stated for 24 hours with the combination of estrogen and inhibitors prior to collection of conditioned media. Conditioned media taken from cells was used in an in vitro tubulogenesis assay in which secreted factors from TG1-1 cells treated with estrogen lead to HUVEC tubulogenesis. This phenotypic change was abrogated by Fulvestrant, and more strikingly by YC-1 and cycloheximide (B). Conditioned media from breast cancer cells grown under hypoxic conditions for 24 hours demonstrated similar effects (C).

Back to article page