Figure 1

Expressed CD44 isoforms in colorectal cancer. Agarose gel electrophoretogram of PCRs with primer combination A-B, A-D, A-E, C-B, C-E on human CRC cell line HT29. (1. 100bp DNA ladder; 2. CD44 expression pattern of HT29 suspension). Primer design shown on Figure 2A. Expressed CD44 isoforms in colorectal cancer were detected. PCR with A-B primer pair can theoretically provide all expresed isoforms, but for the reason of sensitivity limitations of PCR technique only five main bands were detected. PCRs with the other four primer pairs could reveal expressed (quantitatively inferior) isoforms containing v3 and v6 exon products.Some of the bands could be identified as distinct isoform products (proven by direct sequencing), others covered more, similar length isoforms identified by allele-specific next-generation sequencing or estimated by length calculation of the products. Estimated/calculated isoforms are shown in Italic, red letters. Twenty-six CD44 isoforms could be differentiated this way, some of them are just truncated, so that may in fact cover several isoforms sharing the same common variant exons.