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Figure 5 | Molecular Cancer

Figure 5

From: Ovarian cancer ascites increase Mcl-1 expression in tumor cells through ERK1/2-Elk-1 signaling to attenuate TRAIL-induced apoptosis

Figure 5

Elk-1 activation is essential for the control of Mcl-1 expression. (A) OVCAR3 cells were transfected with vehicule (control), control siRNA luciferase (siRNA Luc) or Elk-1 specific siRNA (siRNA Elk-1) and 24 h later cells were treated with OVC415 ascites for 1 h or left in medium with FBS. Lysates were obtained 1 h after the stimulation with ascites and immunoblots were probed with anti-phospho-Elk-1, anti-Elk-1 and anti-Mcl-1 antibodies. Tubulin was used as a loading control. (B) CaOV3 cells were transfected with vehicule, control siRNA Luc or Elk-1 specific siRNA and 24 or 48 h later cells were stimulated with either OVC415 (left panel) or OVC439 ascites (right panel). Lysates were obtained and immunoblotted with anti-Elk-1, anti-Mcl-1, anti-phospho-Elk-1 and anti-tubulin. Representative data from three independent experiments.

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