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Figure 6 | Molecular Cancer

Figure 6

From: Ovarian cancer ascites increase Mcl-1 expression in tumor cells through ERK1/2-Elk-1 signaling to attenuate TRAIL-induced apoptosis

Figure 6

Ascites-induced ERK1/2 activation is independent from the activation of FAK. (A) Immunoblot analysis of expression and activation of FAK in both CaOV3 and OVCAR3 cells in the presence of ascites (OVC415). (B) Cells were transfected with vehicle (control), control luciferase siRNA (siRNA Luc) or FAK siRNA (siRNA FAK) and 24 h later cells were stimulated with OVC415 ascites for 1 h. Lysates were then obtained and analyzed by immunoblot for total and phospho-FAK (Tyr397), phospho-Akt (Ser473), phospho-ERK1/2, phospho-Elk-1 and Mcl-1. Representative data from three independent experiments.

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Molecular Cancer

ISSN: 1476-4598