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Figure 1 | Molecular Cancer

Figure 1

From: Combined targeting of AKT and mTOR synergistically inhibits proliferation of hepatocellular carcinoma cells

Figure 1

Feedback activation of AKT after mTOR inhibition is time- and dose dependent. (A) HCC cell lines Hep3B, HepG2 and Huh7 were treated for 24 h with increasing concentrations of RAD001 as indicated. Phosphorylation status of mTOR and AKT was analyzed by Western blot with phospho specific antibodies. (B) Dose dependent expression of pAKT (S473) and pAKT (T308) after 24 h treatment with RAD001 was quantified from three independent experiments as shown in A. Columns, mean percentage of three independent experiments; bars, SD, * p < 0.05. (C) Huh7 and (D) HepG2 cells were treated with 100 nM RAD001 up to 72 h, and cell lysates were prepared at the indicated time points. Where indicated, medium was removed after 48 h and replaced by fresh, 100 nM RAD001 containing medium. HSC70 served as loading control.

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Molecular Cancer

ISSN: 1476-4598