RAD001 and MK-2206 synergistically suppress subcutaneous tumor growth in vivo. Huh7 cells were injected subcutaneously into SCID mice (n = 7 per group). After formation of palpable tumors, mice were treated with Placebo, RAD001, MK-2206 or the combination of both, respectively. (A) Treatment with RAD001 and MK-2206 significantly prolonged survival of tumor bearing mice compared to all other groups (p < 0.05). (B) Tumor volume was monitored over an 18 day period, presented as mean ± SEM. RAD + MK vs Placebo and RAD + MK vs MK-2206 alone was significant at day 15 (p < 0.05). Note that one mouse had to be withdrawn from the experiment in the RAD001 and MK-2206 treatment group at day 13 and 15 respectively, and two mice were withdrawn from the Placebo group at days 11 and 15. (C) AKT and mTOR signaling in tumor samples was analyzed by Western blot.