Figure 3
From: IL-1β promotes stemness and invasiveness of colon cancer cells through Zeb1 activation
IL-1β-induced sphere cells express stem cell markers and Zeb1. (A) Transcription levels of stem cell markers in control and IL-1β-treated colon cancer cells. HCT-116 and HPCC cells were cultured in SFM with or without IL-1β for seven days. mRNA levels of stem cell markers were determined by real-time PCR. β-actin was used as an internal normalization control. Error bars represent SEM. *p < 0.05. (B) Immunoblot analysis for Bmi1 and Nestin from control and IL-1β-treated cells. β-actin was used as an sample loading control. (C) IL-1β-induced sphere cells develop drug resistance. The control and IL-1β-induced sphere cells were treated with various concentrations of carboplatin for two days. Then, cells were dissociated and stained with Trypan blue, and counted under a microscope. The viability was determined by the percentage of live cells over the sum of live and dead cells. *p < 0.05, **p < 0.01. (D). Transcription levels of Zeb1 in control and IL-1β-treated colon cancer cells. HCT-116 and HPCC cells were cultured in SFM with or without IL-1β for seven days. mRNA levels of Zeb1 were determined by real-time PCR. β-actin was used as an internal normalization control. Error bars represent SEM. *p < 0.05. (E). Immunoblot analysis for Zeb1 from control and IL-1β-treated cells. β-actin was used as an sample loading control. Normalized quantification of band intensities on immunoblots for Zeb1 is listed under each band.