Knockdown of Zeb1 inhibits IL-1β-induced EMT in HCT-116 cells. (A) Zeb1 knockdown cells still maintain epithelial appearance seven days after treatment with IL-1β. Scale bar = 200 μm. Scramble and shZeb1 HCT-116 cells were cultured in the medium with 1% FBS in the presence or absence of IL-1β for seven days. (B) Zeb1 knockdown inhibits IL-β-induced suppression of E-cadherin expression. Immunoblot analysis for Zeb1 and E-cadherin was performed on lysates from control and IL-1β-treated cells. β-actin was used as an sample loading control. Normalized quantification of band intensities on immunoblot for Zeb1 and E-cadherin is listed under each band.