Expression analyses of pediatric liver cancers by real-time PCR. The relative IGF2 (A) PLAG1 (B), IGFBP3 (D), and TIA1 (E) mRNA expression levels of the 45 pediatric liver tumor cases and 7 normal liver samples were determined. The dots represent relative candidate gene expression in relation to the house-keeping gene TBP. Data from the HB and pediatric HCC cases are depicted as black and orange diamonds, respectively. Median expression values are given as red horizontal lines. Correlation analyses of pediatric liver cancers. The mRNA expression levels of IGF2 (C) and IGFBP3 (F) were correlated with PLAG1 and TIA1, respectively. Dots represent log2 values of the relative expression of candidate genes blotted versus each other in the 45 pediatric liver tumor samples (rho = Spearman's rank correlation coefficient).