Deficiency of CCAAT/enhancer binding protein family DNA binding prevents malignant conversion of adenoma to carcinoma in NNK-induced lung carcinogenesis in the mouse

Table 2 Tumor multiplicities/incidences in NNK-treated A-C/EBP- or A-C/EBP+ mice fed Dox or regular diet at 52 weeks post-NNK administration for additional 10 weeks and analyzed at 62 weeks post-NNK administration

Group	Genotype (*A-C/EBP*	Treatment	Diet during	No. of mice	Body weight	Gross tumor		Histological	lesions		Tumor incidence
	+ or -^a)		52-62 weeks	examined	(g)	per mouse	Alveolar hyperplasia per mouse	Adenoma per mouse	Carcinoma per mouse	Adenoma and carcinoma per mouse	(%)^b
1	-	NNK	Dox	19	42.2±1.4^d	2.00	0.37	1.63	0.37^e	2.00	95 (17)^c
2	+	NNK	Dox	18	44.5±1.5^d	2.83	0.33	2.06	0.056^f	2.11	83 (15)
3	-	NNK	Regular	18	36.2±1.6	1.89	0.11	1.50	0.44	1.94	94 (16)
4	+	NNK	Regular	18	38.1±1.6	2.22	0.17	2.00	0.44	2.44	78 (14)

^aA-C/EBP- is considered as wild-type control.
^bPercentage of mice with adenomas or carcinomas.
^cNumbers in parentheses show the number of mice having tumors.
^dSignificantly different from their respective regular diet group (group 1 vs. 3, 2 vs. 4) at P<0.01 by student t-test.
^eSignificantly different from their respective A-C/EBP+ group (group 1 vs. 2) at P<0.05 by student t-test.
^fSignificantly different from their respective regular diet group (group 2 vs. 4) at P<0.05 by student t-test.

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