Figure 2

Cleavage kinetics. (A) Time course (0.25-30 minutes) of the cleavage reaction between the CL14/CP25 suicide substrate, shown at the top of the figure and the purified wild type (lanes 1–9), and Glu710Gly mutant (lanes 10–18). In lane 19 the protein has not been added. CL1 represents the DNA strand cleaved by the enzymes at the preferred cleavage site, indicated by an arrow. (B) Percentage of cleaved suicide substrate, normalized to the maximum value of the wild type, plotted against time for the reaction with wild type (black) and Glu710Gly mutant (grey). Data shown are means ± SD from 3 independent experiments.