Over-expression of miR-204 results in loss of Mcl-1 and causes cell death in pancreatic cancer cells. A. Target Scan prediction of the miR-204 pairing site in Mcl-1 3’UTR. B. Left Panel. Pancreatic cancer cells show a decreased expression of miR-204, compared to HPDEC. Data represented as fold-change vs. HPDEC cells. miR-204 expression was measured by Real-Time PCR. Right Panel. Over-expression of miR-204 in MIA PaCa-2 and S2-VP10 cells transfected with a miR-204 mimic. C. Over-expression of miR-204 results in loss of cell viability in both MIA PaCa-2 and S2-VP10 cells. The bars represent mean ± standard error of the mean, n = 3. *p <0.05 (TR: transfection reagent only). D. Over-expression of a miR-204 mimic in MIA PaCa-2 and S2-VP10 cells results in reduced Mcl-1 protein expression 48 h post-transfection. β-Actin is used as a loading control (NT: non-transfected TR: transfection reagent only).