miR-204 binds directly to the 3’ UTR of Mcl-1. A. Luciferase activity in MIA PaCa-2 cells transiently transfected with the luciferase construct alone, or co-transfected with the vector containing either wild type or mutant miR-204 described in A. Mean ± SEM, *p < 0.01. B. Alignment of miR-204 with the predicted target region in the Mcl-1 3’UTR. The binding site deletion used to establish direct binding between miR-204 and Mcl-1 is shown. All constructs were cloned into the Psicheck2 vector.