Cell binding and internalisation screens. (A) Images, from the 8200 Cellular Detection System, for cell binding and non-binding scFvs on MDA-MB-231 cells. Binding of each scFv to MDA-MB-231 cells was detected with a murine anti-HIS antibody and an Alexa647 anti-mouse antibody. (B) Images, from the 8200 Cellular Detection System, of an internalising scFv and a non-internalising scFv obtained from the CypHer5e internalisation screen on MDA-MB-231 cells. (C) Effect on MDA-MB-231 cell viability of 22 hit hybridomas able to internalise and deliver a toxic secondary antibody conjugated to saporin.