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Figure 7 | Molecular Cancer

Figure 7

From: Group X secreted phospholipase A2 induces lipid droplet formation and prolongs breast cancer cell survival

Figure 7

hGX alters the expression levels of genes involved in lipid metabolism. MDA-MB-231 cells were grown in complete culture medium in the presence of hGX (1 nM) for 48 h. The medium was removed and cells were washed and incubated for an additional 24 h in serum-free and hGX-free medium containing 0.02% FAF BSA. Cell lysates were prepared at the time points indicated and the relative expression levels of genes involved in lipid metabolism were determined by means of qPCR (A). The relative protein expression levels of ACC1 and VLCAD were analyzed by immunoblotting (B) at the indicated time-points. (C) Protein band intensities were analyzed by densitometry and normalized to loading control (β-actin) values at each time-point. Values on the graphs are means ± SD of three experiments performed in duplicate and results that are statistically significant over control samples are indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001; one-way ANOVA with Bonferroni adjustment).

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