Figure 3

The expression of E-Cadherin in the presence of integrin inhibitors. (A) Western blot and densitometric analysis of E-Cadherin expression in RWPE1, PC3, HS5 and co-cultured cells (PC3 + HS5) in the presence and absence of integrin inhibitors. (B) Immunostaining of E-Cadherin in PC3 cells. (B’) Immunostaining of E-Cadherin in PC3 cells in the presence of α6 and β1 inhibitors. (C-C’) Immunostaining of E-Cadherin in HS5 cells. (D) Expression of E-Cadherin in HS5 cells in the presence of α6 and β1 inhibitors. (E-E’) Immunostaining of E-Cadherin (red fluorescence) was primarily expressed by HS5 cells (STRO-1; filled arrowhead) in co-culture. (F-F’) Expression of E-Cadherin in co-cultured cells in the presence of α6 and β1 inhibitors saw an up-regulation on HS5 cells (STRO-1; filled arrowheads) and a re-expression of E-Cadherin on PC3 cells (Cell Mask; unfilled arrowheads). Scale bars = 40 μm.