Figure 3

LBH589 inhibits invasive potential of HCC cells in vitro . (A) Cell invasion experiment results showed LBH589 significantly inhibited the invasive capacity of HCC-LM3 and HepG2 cells, and gankyrin overexpression attenuated the LBH589-induced inhibition of invasion in HCC-LM3 and HepG2 cells. ***P < 0.001. The results represent means ± SD of experiments performed in triplicate. (B) Western blotting showed LBH589 increased the expression of E-cadherin and decreased the expression of N-cadherin, vimentin, VEGF and TWIST1 in HCC-LM3 and HepG2 cells. Overexpression of gankyrin abrogated the effect of LBH589-induced reduction of EMT and angiogenesis. β-actin was used as the internal control. All assays were done in triplicate. (C) Single and merged images were taken to show immunofluorescence staining of N-cadherin (green) and vimentin (red) accompanied by the cell nucleus (blue) stained by DAPI.