LBH589 inhibits proliferation and metastasis of HCC in vivo . (A) Photomicrographs were taken for liver tumors in nude mice, 5 weeks after orthotopic xenograft transplantation of HCC-LM3, vector ctrl transfected or gankyrin transfected HCC-LM3 cells. Representative images of a mouse in each group were presented. Tumor volumes from each group were measured as described in Materials and Methods. (B) Representative lung tissue sections from each group were shown (hematoxylin and eosin stain; magnification, × 100). The number of lung metastatic foci in each group was calculated. (C) Tumors from different groups were immunostained for indicated molecules. CD31-stained microvessels were counted to record microvessel density; Apoptosis cells were counted to give the apoptosis index; and cells expressing Ki-67 were counted to calculate the proliferation index. Pictures are representative of three independent experiments. Black scale bars, 100 μm. (D) Western blotting was performed to detect expression of indicated molecules in tumor samples. β-actin was used as the internal control. All assays were done in triplicate. *P < 0.05, the results expressed as means ± SD of three independent experiments.