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Figure 1 | Molecular Cancer

Figure 1

From: Reversal of chemosensitivity and induction of cell malignancy of a non-malignant prostate cancer cell line upon extracellular vesicle exposure

Figure 1

Extracellular vesicle-mediated reversal of apoptosis resistance and sensitivity. A, B. EVs were isolated from DU145 and RC1 cells. The EVs were resuspended in PBS. DU145 cells were co-cultured with RC1 EVs (REV) and RC1 cells were co-cultured with DU145 EVs (DEV). Non-EV and EV co-cultured cells were treated with 100 nM CPT for 24 h and examined for PARP cleavage and actin via Western blot analysis. Proteins (unless indicated 50 μg/sample was used for Western blot analysis) were separated by 10% SDS-PAGE, transferred to nitrocellulose and analyzed with antibodies to the indicated proteins. C,D. The same experiment from B,C was repeated and the samples were examined for apoptosis via propidium iodide staining using a flow cytometer. The data is the mean +/− s.d. of 2 independent experiments performed in triplicate. Note: for all Western blots described in this Figure legend and for all other subsequent Figure legends, the exposure time used to identify the various proteins was variable.

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