Figure 5

Detection of proteins from patients EVs. A. EVs were isolated from conditioned medium from tissue biopsied from 2 patients as described in Experimental procedures. EVs were co-cultured with PrECs for 7 days. A portion of the sample was used for mass spec analysis while the other for Western blot analysis. We examined the expression of pRKIP, RKIP, 14-3-3 zeta and actin based on the Uniprot ID data (Table 2). B. Venn diagram comparing the protein content of PrECs alone vs. PrECs co-cultured with Patient 18 EVs (8 proteins were unique to PrECs alone, 16 proteins were unique to PrECs co-cultured with Patient 18 EVs, and 28 proteins were found in common). C. Venn diagram comparing the protein content of PrECs alone vs. PrECs co-cultured with Patient 19 EVs (8 proteins were unique to PrECs alone, 15 proteins were unique to PrECs co-cultured with Patient 19 EVs, and 28 proteins were found in common). D. Venn diagram comparing the protein content of PrECs co-cultured with Patient 18 EVs vs. PrECs co-cultured with Patient 19 EVs (44 proteins were unique to PrECs co-cultured with Patient 18 EVs, 43 proteins were unique to PrECs co-cultured with Patient 19 EVs, and 38 proteins were found in common).