¹H NMR-based metabolic profiling of human rectal cancer tissue

Wang, Huijuan; Wang, Liang; Zhang, Hailong; Deng, Pengchi; Chen, Jie; Zhou, Bin; Hu, Jing; Zou, Jun; Lu, Wenjie; Xiang, Pu; Wu, Tianming; Shao, Xiaoni; Li, Yuan; Zhou, Zongguang; Zhao, Ying-Lan

doi:10.1186/1476-4598-12-121

Molecular Cancer

Table 2 Metabolite changes between each stage of rectal cancers and normal controls

From: ¹H NMR-based metabolic profiling of human rectal cancer tissue

Metabolites	chemical shift	Mutiplicity^a	Normal control vs. I			Normal control vs. II			Normal control vs. III			Normal control vs. IV
Metabolites	(ppm)	Mutiplicity^a	VIP^b	P-value^c	FC^d	VIP^b	P-value^c	FC^d	VIP^b	P-value^c	FC^d	VIP^b	P-value^c	FC^d
Myo-inositol	4.06	t	3.04	<0.001	-1.90	3.35	<0.001	-1.93	3.99	<0.001	-2.17	3.16	<0.001	-2.07
	3.55	dd	2.92	<0.001	-1.95	3.27	<0.001	-1.99	3.89	<0.001	-2.20	3.09	<0.001	-2.08
	3.63	t	2.35	<0.001	-2.00	2.71	<0.001	-2.04	3.42	<0.001	-2.36	2.80	<0.001	-2.42
Taurine	3.27	t	3.10	<0.001	-2.01	3.37	<0.001	-2.03	3.99	<0.001	-2.28	3.18	<0.001	-2.10
α-Glucose	3.55	dd	2.92	<0.001	-1.95	3.27	<0.001	-1.99	3.89	<0.001	-2.20	3.09	<0.001	-2.08
	5.23	dd	2.60	<0.001	-4.75	2.81	<0.001	-5.58	3.07	<0.001	-4.57	2.44	<0.001	-4.97
Phosphocreatine	3.93	s	3.02	<0.001	-2.34	3.10	<0.001	-2.07	3.50	<0.001	-2.09	2.91	<0.001	-2.02
	3.04	s	2.74	<0.001	-2.29	2.94	<0.001	-2.15	3.36	<0.001	-2.16	2.66	<0.001	-1.71
Creatine	3.94	s	3.02	<0.001	-2.34	3.10	<0.001	-2.07	3.50	<0.001	-2.09	2.91	<0.001	-2.02
	3.04	s	2.74	<0.001	-2.29	2.94	<0.001	-2.15	3.36	<0.001	-2.16	2.66	<0.001	-1.71
Glycolate	3.93	s	3.02	<0.001	-2.34	3.10	<0.001	-2.07	3.50	<0.001	-2.09	2.91	<0.001	-2.02
Betaine	3.89	s	1.97	<0.001	-1.85	2.56	<0.001	-2.10	2.57	<0.001	-1.88	1.88	<0.001	-1.62
Dimethylglycine	3.71	s	2.21	<0.001	-2.58	2.33	<0.001	-2.60	2.39	<0.001	-2.13	1.98	<0.001	-2.22
Glyceryl	4.3	m	2.32	<0.001	-1.48	2.40	<0.001	-1.46	2.59	<0.001	-1.45	1.98	<0.001	-1.38
Lactate	1.33	d				1.23	<0.001	1.27	1.84	<0.001	1.26	2.26	<0.001	1.22
	4.11	q				1.13	0.004	1.73	1.52	0.001	1.88	1.52	0.001	2.22
Threonine	1.33	d	0.08	<0.001	1.21	1.23	<0.001	1.27	1.84	<0.001	1.26	2.26	<0.001	1.22
Acetate	1.93	s							2.65	<0.001	6.95	1.61	<0.001	2.96
Glutathione	2.56	m	2.49	<0.001	1.62	2.41	<0.001	1.60	2.30	<0.001	1.46	2.35	<0.001	1.81
	2.96	m							1.84	<0.001	1.42	1.50	<0.001	1.39
Uracil	7.54	d	1.99	<0.001	3.04	2.58	<0.001	3.69	2.33	<0.001	2.94	2.33	<0.001	2.46
	5.8	d	0.91	<0.001	3.06	1.84	<0.001	3.60	2.12	<0.001	3.45	2.19	<0.001	2.67
Succinate	2.41	s	1.05	0.004	1.48	1.15	0.005	1.68	1.93	<0.001	1.75	2.08	<0.001	3.03
O-acetyl glycoprotein	2.07	s							2.30	<0.001	2.65	1.30	0.033	1.35
Mannitol	3.69	m	1.31	0.015	-1.20	1.79	<0.001	-1.32	1.53	0.005	-1.23	1.48	<0.001	-1.46
	3.88	m	1.44	<0.001	-1.31	1.68	<0.001	-1.36	1.37	0.004	-1.25	1.04	0.026	-1.24
Dimethylamine	2.73	s							1.24	0.069	1.16	1.97	<0.001	2.32
Leucine	0.96	t							1.22	0.004	1.19	1.73	<0.001	1.30
Valine	1.05	d										1.47	<0.001	3.29
	0.99	d				1.84	0.011	1.31	1.59	0.023	1.37	1.46	<0.001	1.93
β-hydroxybutyrate	1.2	d	1.10	0.613	-1.07	1.55	<0.001	2.75	2.49	<0.001	2.68	1.27	0.010	1.53
Glutamine	2.14	m							1.34	0.0011	1.50	1.43	0.003	1.75
	3.77	m				1.91	<0.001	1.53	1.43	<0.001	1.31	0.87	0.097	1.18
Acetoacetate	2.28	s										1.90	<0.001	1.55
Sarcosine	2.75	s										1.58	<0.001	2.15
Tyrosine	7.2	d	1.74	0.016	-1.38	1.64	0.012	-1.43	1.42	0.051	-1.28	1.31	0.004	-1.95
	6.9	d	1.61	0.277	-1.21	1.40	0.275	-1.20	1.29	0.269	-1.21	1.22	0.006	-2.55
Alanine	1.48	d				1.69	0.010	1.59	1.59	0.014	1.81	1.00	0.022	2.79
Acetoacetic acid	2.31	s										1.77	<0.001	1.60
Serine	3.98	m	1.31	<0.001	1.49	1.93	<0.001	1.56	1.58	<0.001	1.38	1.63	0.004	1.29
Isoleucine	1.01	d										1.16	0.006	1.54
	0.95	t										1.46	0.019	1.21
Trimethylamine-N-oxide	3.27	s				1.60	<0.001	3.59	1.23	<0.001	2.73	1.67	0.008	2.09
Lysine	3.77	m				1.91	<0.001	1.53	1.43	<0.001	1.31
Acetone	2.23	s										1.34	0.003	1.49
PC(phosphochline)	3.21	s				1.68	<0.001	1.31	1.13	0.017	1.19	1.40	0.003	1.22
GPC(glycerophosphochline)	3.23	s	2.00	<0.001	1.49	1.75	<0.001	1.51	1.14	0.002	1.26	1.92	<0.001	1.35
NAD	8.83	d										1.05	0.015	3.25
	9.15	d										1.55	<0.001	1.97
2-Hydroxyisobutyric acid	1.44	s	1.00	0.018	-1.18	1.17	0.007	-1.22	1.23	0.012	-1.20
Trytophan	7.29	m	1.35	0.012	-1.32	1.75	<0.001	-1.50
Formic acid	8.44	s										1.53	<0.001	1.93

^aMultiplicity: s singlet, d doublet, t triplet, q quartet, dd doublet of doublets, m multiplet.
^bVariable importance in the projection was obtained from OPLS-DA model with a threshold of 1.0.
^cp-value obtained from Student’s t-test.
^dFold change(FC) between rectal cancers and normal controls. Fold change with a positive value indicates a relatively higher concentration present in rectal cancer patients while a negative value means a relatively lower concentration as compared to the normal controls.

Back to article page

ISSN: 1476-4598

Contact us

General enquiries: journalsubmissions@springernature.com