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Figure 4 | Molecular Cancer

Figure 4

From: Targeted therapy against EGFR and VEGFR using ZD6474 enhances the therapeutic potential of UV-B phototherapy in breast cancer cells

Figure 4

ZD6474 modulates UV-B action by altering the expression of caspases and apoptotic proteins. Time vs. pNA formation graphs of (A) MCF-7 and (B) MDA-MB-468 treated ZD6474 (ZD) and/or UV-B radiation for 48 h was studied by monitoring the spectrophotometric readings at 405 nm in order to study the Enzyme kinetics of caspase-3/7 using Ac-DEVD-pNA as substrate. Specific activity of Caspase-3/7 was studied for (C) MCF-7 and (D) MDA-MB-468. Bars (mean ± S.E, n = 3), and * (p < 0.05), ** (p < 0.01) represent levels of significance with respect to control. Western blotting of (E) MCF-7 and (F) MDA-MB-468 cells treated with ZD6474 (Z) and/or UV-B (R) for 48 h and probed with anti-PARP, cyclin E, caspase-3, casapse-7, bcl-2, E-cadherin. β-actin protein expression was used as an internal probe for equal loading. Representative of three independent experiments. Intact PARP, complete arrow; cleaved PARP, dashed arrow; block filled arrow, caspase-3 (MDA-MB-468).

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